2× primary antibody staining solution (1:400 final) (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
2× primary antibody staining solution (1:400 final)
2× Primary Antibody Staining Solution (1:400 Final), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2%C3%97+primary+antibody+staining+solution+(1%3A400+final)/2%C3%97+primary+antibody+staining+solution++1+400+final+/pmc12089343-343-85-99
Average 90 stars, based on 1 article reviews
2× Primary Antibody Staining Solution (1:400 Final), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2%C3%97+primary+antibody+staining+solution+(1%3A400+final)/2%C3%97+primary+antibody+staining+solution++1+400+final+/pmc12089343-343-85-99
Average 90 stars, based on 1 article reviews
2× primary antibody staining solution (1:400 final) - by Bioz Stars,
2026-09
90/100 stars
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Incubation:Article Title: Transcriptional and epigenetic rewiring by the NUP98::KDM5A fusion oncoprotein directly activates CDK12 Article Snippet: 1 × 10 6 cells were harvested, washed with PBS and stained with Zombie Aqua Fixable Viability Dye (1:1000, BioLegend, USA) for 10 minutes. .. After PBS wash, cells were fixed with 2% phosphate-buffered formaldehyde solution (Roti-Histofix 4,5%, Carl Roth, Germany) for 15 min. After washing with PBS, cells were permeabilized with 0.2% TritonX-100 (PanReac AppliChem, Germany) in PBS supplemented with 10% FBS for 15 min, followed by incubation in 0.1% TritonX-100 in PBS supplemented with 10% FBS for 30 min. Next, cells were incubated with anti-mouse CD16/CD32 antibody (1:200, Mouse BD Fc Block, clone 2.4G2, BD Biosiences, Germany) for 10 min, followed by the direct addition of the 2× Blocking Assay:Article Title: Transcriptional and epigenetic rewiring by the NUP98::KDM5A fusion oncoprotein directly activates CDK12 Article Snippet: 1 × 10 6 cells were harvested, washed with PBS and stained with Zombie Aqua Fixable Viability Dye (1:1000, BioLegend, USA) for 10 minutes. .. After PBS wash, cells were fixed with 2% phosphate-buffered formaldehyde solution (Roti-Histofix 4,5%, Carl Roth, Germany) for 15 min. After washing with PBS, cells were permeabilized with 0.2% TritonX-100 (PanReac AppliChem, Germany) in PBS supplemented with 10% FBS for 15 min, followed by incubation in 0.1% TritonX-100 in PBS supplemented with 10% FBS for 30 min. Next, cells were incubated with anti-mouse CD16/CD32 antibody (1:200, Mouse BD Fc Block, clone 2.4G2, BD Biosiences, Germany) for 10 min, followed by the direct addition of the 2× Staining:Article Title: Transcriptional and epigenetic rewiring by the NUP98::KDM5A fusion oncoprotein directly activates CDK12 Article Snippet: 1 × 10 6 cells were harvested, washed with PBS and stained with Zombie Aqua Fixable Viability Dye (1:1000, BioLegend, USA) for 10 minutes. .. After PBS wash, cells were fixed with 2% phosphate-buffered formaldehyde solution (Roti-Histofix 4,5%, Carl Roth, Germany) for 15 min. After washing with PBS, cells were permeabilized with 0.2% TritonX-100 (PanReac AppliChem, Germany) in PBS supplemented with 10% FBS for 15 min, followed by incubation in 0.1% TritonX-100 in PBS supplemented with 10% FBS for 30 min. Next, cells were incubated with anti-mouse CD16/CD32 antibody (1:200, Mouse BD Fc Block, clone 2.4G2, BD Biosiences, Germany) for 10 min, followed by the direct addition of the 2× |